Coding

Part:BBa_K4429006:Design

Designed by: Anish Mulchandani   Group: iGEM22_IISER-Pune2_India   (2022-07-13)


Ab513 IgG1k LC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ensuring the correct V->C region interface. Used Uniprot IMGT entry to find where constant region starts.

Removing BioBrick illegal restriction sites using silent mutation in SnapGene.


Source

Variable region protein sequence from PDB 5AAW [1], reverse translated using E. coli codon usage table in SnapGene, constant region from IMGT [2]. The entire light chain was then codon optimised for E. coli K12 using IDT codon optimisation tool.

References

[1] https://www.rcsb.org/structure/5AAW

[2] https://www.imgt.org/ligmdb/view?id=X51755